LMB™ (Liposomal Methylene Blue) Stick Packs – NOT READY YET, IN PRE-PRODUCTION NOW, ESTIMATE DATE THE PRODUCT WILL BE READY AROUND FEB 15TH, 2023

LMB^TM – Liposomal Methylene Blue Supplement

Table of Contents

1. Overview

2. How does Methylene Blue work in the brain?

3. How Aging Kills Us

4. Methylene Blue benefits

• Methylene Blue boosts acetylcholine
• Methylene Blue is an antidepressant
• Methylene Blue resists Alzheimer’s Disease
• Methylene Blue is anti-aging
• Methylene Blue improves memory

5. How does Methylene Blue feel?

6. Methylene Blue Clinical Research

• Methylene Blue Improves Memory
• Methylene Blue helps with Bipolar Disorder

7. Methylene Blue Recommended Dosage

8. What Are Liposomal Supplements

9. Liposomal Supplements Manufacturing Process

10. Liposomal Supplements Production Overview

Overview

Methylene blue was synthesized in 1876 by a German chemist named Heinrich Caro. This is when it became a fabric dye used in the textile industry. Additionally, it also became popular as a bacteriologic stain, a cellular stain, an indicator dye, and for surgical and medical marking.  But even back then, people started to use it to treat medical conditions like malaria. Later in the early 20th century, scientists realized some of its potential and started to use it experimentally – to treat symptoms of schizophrenia. Nowadays, we are studying the potential of using methylene blue to treat many other neurodegenerative disorders like Alzheimer’s and Parkinson’s disease. These studies have shown methylene blue to be a potential therapy for mild cognitive impairment. The diseases that seem to show a response to methylene blue are often linked with mitochondrial dysfunction. This is said to be because brain cells and neurons depend almost completely on mitochondrial-based energy. Of course, mitochondrial failure can affect the rest of your body too but the effects are the most prominent on the brain tissue.

Methylene Blue (methyl thioninium chloride) is basically a synthetic compound manufactured in a lab and classified as a nootropic. This means it’s linked to improvement in mood, longevity, and memory. Some research has also shown that has anti-aging properties. Consequently, Methylene Blue is dubbed to be one of the most critical nootropics to be effective against aging and neurological diseases available today. Nootropics have a special structure that allows them to cross the blood-brain barrier quickly and efficiently. Once the barrier is crossed it acts directly on the mitochondria to improve the efficiency of respiration – acting as an antioxidant. This in turn increases the brain lifespan.

Methylene Blue is a unique compound, even unique amongst its class of Nootropics. Unlike other nootropics which act by increasing the synthesis of neurotransmitters and in turn neural signaling, methylene blue improves brain function, in particular memory, by increasing cerebral blood flow and therefore increasing respiration.

Studies show dramatic increases in cellular oxygen consumption and glucose uptake when using Methylene Blue. MB increases CMRO2 (cerebral metabolic rate) through increased activity in the mitochondrial electron transport chain.

Numerous research articles have documented the dramatic increase in oxygen consumption and consequently glucose intake through the use of Methylene Blue. These articles have deduced that Methylene Blue increases oxygen availability simply by increasing cerebral metabolic rate through the process of increased mitochondrial activity.

Specifically, it increases the process of the electron transport chain within the mitochondria. It does this by functioning as an alternative electron carrier. This unique process allows it to transfer electrons from one compound to another (from NADH to cytochrome c).

Another property of methylene blue is that it also stimulates glucose uptake. This additive factor along with increased cerebral oxygen flow is what helps it increase glucose turnover and in turn ATP production.

Increased ATP production has several benefits, but broadly, more ATP means more cellular energy. More cellular energy is what betters the overall brain functioning, including functions like cognition, mood, and even memory.

Another unique property gives Methylene Blue a neuroprotective effect. Methylene Blue is an effective antioxidant compound. There is a great need for antioxidants in the brain as the byproduct of mitochondrial reaction includes reactive oxygen species. The free radicals are usually formed inside the cell and are termed ‘Superoxide’. Methylene Blue acts by simply attaching to these free radicals and forming water, which is a completely harmless compound. Superoxide free radicals are produced during the first stage of mitochondrial reactions which means methylene blue stops the oxidative cascade before it gets a chance to commit damage.

Therefore, it can be said that methylene blue has dual properties – it increases energy reserves within the cell and eliminates the oxidative stress caused by the energy turnover system. It’s a metabolic enhancer as well as an antioxidant.

Before methylene blue was used for neurodegenerative disorders researchers used a compound called rotenone. Rotenone is a potent pesticide, it worked on neurodegenerative disorders by causing severe dopamine depletion in areas of the brain that caused Parkinson’s Disease.

Of course, when methylene Blue was discovered, it quickly took over Rotenone’s place as it protected the mitochondria from the damaging effect of Rotenone. It did this by acting as an alternative electron carrier and also countering cerebral ischemia and in turn reperfusion damage. Reperfusion damage is the destruction of tissue when oxygenated blood returns to a previously ischemic area. This usually occurs during an ischemic stroke or traumatic brain injury. Methylene blue successfully counteracts the neuropathological, behavioral, and neurochemical symptoms caused by Parkinson’s disease.

Methylene Blue helps:

Neurotransmitters: Some important neurotransmitters in the brain include catecholamines and acetylcholine. Certain enzymes, namely monoamine oxidase, and acetylcholinesterase inhibit the production of these neurotransmitters. Methylene Blue stops this action and consequently increases the catecholamines and acetylcholine. It is also linked to an increase in serotonin and norepinephrine which usually affect levels of anxiety, depression, and memory.

Mitochondrial Energy: Methylene Blue increases the rate of respiration in the brain by increasing oxygen availability. It also encourages a process that donates electrons to an electron transport chain within the mitochondria. The same process takes place to derive energy from the food you eat. Therefore, methylene blue becomes responsible for the energy production process in the brain. It basically takes over as a source of energy that was previously supplied by nutrients from food. Of course, increasing cellular energy has an exponential effect on mood and memory.

Paul Ehrlich, a German Physician who was also a Nobel Prize recipient was the first scientist to use Methylene Blue to treat human conditions. He used it to treat an infection called Malaria.

During his research trials, Ehlirich discovered many properties of methylene blue. He observed that it tends to concentrate in the brain and selectively targets diseased tissue anywhere in the body. His findings are what drove him to dub methylene blue as the ‘Magic Bullet’, a term used up to this day to describe this compound.

Many years have passed since his experimentation and discovery. Nowadays, Methylene Blue is used to treat many diseases like dementia, urinary tract infections, malaria, methemoglobinemia, cyanide, and carbon monoxide poisoning. It is even used as an agent in chemotherapy. New research has shown that methylene blue is responsible for enhancing mitochondrial function, as previously mentioned. It does this by increasing cerebral blood flow and through this process, it has the potential to act as an antidepressant.

How Aging Kills Us

As we age, so does our brain, the brain ages through chemical and metabolic changes. For example, with time decrease in mitochondrial energy causes a decrease in attention, memory, and mental agility. Additionally, some compounds, namely Tau proteins and Amyloid,  accumulate to form plaques that cause clogging of the brain tissue. Oxidative stress from this clogging causes an increase in free radical damage as well as a decline in cerebral blood flow.

These changes most commonly occur in the elderly, but they can also occur at any age. They can be altered by the food we eat, what we drink, how much we drink, lifestyle habits, and even the air we breathe.

Since methylene Blue is an active inhibitor of oxidative stress and it also increases cerebral blood flow it can help decrease the rate of cognitive decline.

Methylene Blue benefits

Research has shown that low-dose Methylene Blue supplementation causes memory-enhancing effects in both animals and humans. It basically functions as an anti-depressant, anti-aging formula that is helpful in diseases like dementia, Huntington’s, and Alzheimer’s.

Other effects of methylene blue supplementation include lowering blood pressure, improving cognition in young and healthy people, boosting mitochondrial function, anti-microbial properties, relieving anxiety, and anti-aging properties on the skin.

Methylene Blue Boosts Acetylcholine

Methylene Blue is an active inhibiter of the enzyme acetylcholinesterase, specifically muscarinic acetylcholine. Acetylcholine is a commonly used neurotransmitter in the brain as well as the spine. Methylene Blue makes sure it is more available in the brain.

Methylene Blue Is An Antidepressant

Methylene Blue is also a monoamine oxidase inhibitor (MAOI). However, it inhibits MAO-A more than MAO-B. But some trials have discovered that it inhibits both at large doses. This property makes it useful in the treatment of psychiatric conditions like Bipolar Disorder and Methylene Blue.

A study conducted in 1987 showed that 15 mg per day of Methylene Blue was a powerful antidepressant in patients that suffered from severe depression.

Another study with 31 Bipolar Disorder trail subjects compared 300 mg per day of Methylene Blue with 15 mg per day. These patients were concurrently on lithium treatment. Lithium is an antipsychotic medication commonly used in the treatment of Bipolar disorder. The results of the study showed that the 300 mg dose of Methylene Blue was a “useful addition to lithium in the long-term treatment of manic-depressive psychosis”. Which can be one of the symptoms of Bipolar Disorder. It was also noted that patients were significantly less depressed.

Methylene Blue resists Alzheimer’s Disease

Alzheimer’s is a neurodegenerative disorder that results in dementia because of a buildup in Taue proteins. Some clinical studies have shown that Methylene Blue is a potent inhibitor of Tau protein buildup. This is why it is under consideration for being listed as a drug to treat Alzheimer’s Disease.

Here are some of the ways that Methylene Blue is linked with treating Alzheimer’s symptoms:

• Methylene blue is an inhibitory action on the cGMP pathway, this pathway is closely related to the progression of Alzheimer’s disease.
• Methylene blue allows neurons to actively resist the formation of tau protein plaques as well as the neurofibrillary tangle.
• Methylene enhances cellular metabolism and increases cerebral perfusion.
• Methylene Blue mediates certain chemical pathways, namely cholinergic, serotonergic, and glutamatergic, which play a critical role in the development of Alzheimer’s disease.

Consequently, Methylene Blue not prevents the disease from occurring but also delays the effect after the diagnosis of Alzheimer’s is made. This is a significant milestone in the treatment of Alzheimer’s disease since previously all the treatments were only focused on the prevention of the disease.

Methylene Blue Is Anti-Aging

Some reliable studies have proven that the Nootropic compound- Methylene Blue does in fact have effective antiaging properties. Some of those properties are listed below:

• As previously mentioned, Methylene Blue is an active participator in the electron transfer chain, compounds that can carry electrons from one compound to another are also known as redox agents. This transfer or cycling of electrons is what helps it block oxidant production in the mitochondria. An enzyme involved in this reaction is called Mitochondrial complex IV and it is the last enzyme in the electron transport chain within the mitochondria. Methylene blue increases the production of this complex by 30%, which means 30% more energy.
• Methylene Blue also increases heme synthesis. Heme is a compound containing iron which is vital for the transport of oxygen from one place to another inside the body. Heme is even used during DNA synthesis. Both of these processes are vital for the healthy turnover of skin cells.
• Another property of Methylene Blue includes reversing premature senescence caused by H2O2 or cadmium. Senescence is basically biological aging or the gradual deterioration of cellular function. It triggers the shortening of DNA in response to oxidative species like the ones mentioned above.

Methylene Blue Improves Memory

Studies carried out on animals have proven that a low dose of methylene blue improves contextual memory. Contextual memory is the term given to the type of memory used to consciously recall a source or circumstance for a specific reason.

Further studies showed that methylene blue is even helpful in memory retention. This means how efficiently you can remember a certain thing or event. This study was conducted on rats. The reason for the increased memory retention was an increase in metabolic energy which is an aforementioned quality of methylene blue. Furthermore, this increase in metabolic energy was useful in areas of the brain that required more energy during discrimination learning which is what distinguished a high-quality memory from a low-quality one. These results occurred from repeated post-training supplementation.

Methylene Blue is a Redox Agent

A redox agent is any compound or element that has the ability to lose and gain electrons. Since methylene Blur acts as an electron donor it can be classified as a redox agent, but more specifically an antioxidant. This property allows it to treat diseases like methemoglobinemia. Its electron losing property allows it to turn the harmful form of hemoglobin- methemoglobin, back into hemoglobin. Methemoglobin is harmful because it loses its ability to carry oxygen, only hemoglobin can carry oxygen which is vital for the functioning of all the cells in our body.

Methylene Blue potently Inhibits Nitric Oxide

Methylene blue inhibits nitric oxide primarily in four ways:

• Displaces the nitric oxide within the mitochondria-when methylene blue attaches to a certain enzyme it allows the displacement of nitric oxide and makes room for the attachment of carbon dioxide.
• Methylene blue acts as a redox agent- the antioxidant properties of methylene blue allow it to absorb the free radicals produced by nitric oxide because of its rapid degradation. Through this action, methylene blue avoids further oxidative stress on the cell.
• Inhibits nitric oxide synthase- methylene blue is also linked to directly inhibiting the production of nitric oxide by inhibiting the enzyme nitric oxide synthase, which is directly involved in the production of nitric oxide.
• It is a monoamine Oxidase Inhibitor (MAOI)- Methylene blue also inhibits the production of nitric oxide through an alternative pathway. In this pathway the enzyme monamine oxidase breaks down neurotransmitters, which also produces nitric oxide, methylene blue inhibits this enzyme consequently decreasing the production of nitric oxide and even contributing to an antidepressant effect as it increases the availability of neurotransmitters through this action.

Methylene Blue exhibits An Antibacterial Effect

Methylene Blue has also been known for its anti-bacterial properties since its discovery. Methylene blue was also used effectively as an antifungal, antiviral, and antibacterial agent before prescription antimicrobials became widely available in the 1950s. Initially, it was used in the treatment of malaria but nowadays its usually used as an alternative treatment for infections of the gut or urine. It’s even used to keep the ecosystem of fish tanks under control with its antifungal properties. However, to exert this antibacterial property in humans it has to be given in an injectable form rather than an oral form, especially when combined with certain frequencies of light.

How Does Methylene Blue Feel?

Even though methylene blue is a nootropic it is unlikely that it will feel different from any other supplementation you have taken before. However, after some time you may start to notice its effects on your brain. The most commonly experienced effect is termed “after the fact learning”. This is meant to feel like your brain automatically sorts through material from your daily life in such an order that you have easy access to it later. Basically, it gives you better recall power.

Furthermore, people taking methylene blue have stated that they tend to forget about negative associations with great ease and only retain positive memories. These people have also said that through this effect they have overcome their social anxiety. Consequently, it has given them a new youth, a new vigor. They tend to feel more focused and confident too.

Additionally, a healthy lifestyle was easier to maintain as Methylene Blue takers have also reported that workouts were easier due to having more energy which is exactly the effect methylene blue has on the powerhouse of the cell- the mitochondria.

Methylene Blue Clinical Research

Methylene Blue has been around for more than a century, which is why it has been the topic of vast research. However, it was only in the last couple of decades that scientists truly figured out how exactly methylene blue alters brain tissue, all the way down to the molecular level in the mitochondria.

Although the main reason behind the occurrence of Alzheimer’s disease is said to be an accumulation of Tau protein plaques, a study published in 2017 investigated other components that could possibly be linked to the diagnosis of Alzheimer’s disease.

Namely, it investigated the energy-making system in the neuronal cells. This process is called glycosylation and occurs in the mitochondria of all cells. It produces energy as a result of respiration. This study found that as humans age mitochondrial metabolism in the normal tissue also deteriorates. This results in an overall decrease in the energy molecules produced. This decrease in energy is linked to the development of neurodegenerative diseases such as Alzheimer’s and even Parkinson’s.

Another study conducted at Children’s Hospital Oakland Research Institute has found a simple solution to this problem. As mentioned before, the decline in energy production is linked to the development of Alzheimer’s. Specifically, the decrease of complex IV. This is where methylene blue comes in. Methylene blue actively slows and even prevents the decline of mitochondrial function by increasing the availability of a compound known as complex IV. Consequently, this increases oxygen consumption and even reverses premature cell death.

Methylene Blue Improves Memory

Methylene Blue has proven to be a potent enhancer of memory. A clinical trial was conducted with 26 healthy volunteers and the purpose was to determine the effect of methylene blue on memory and sustained attention. The results of the study showed that only a single low dose of this compound was needed to increase short-term memory power. It specifically acted on the part of the brain responsible for sensory and visual processing.

Timothy Duong, PhD, the study author concluded Methylene Blue showed promise “in healthy aging, cognitive impairment, dementia and other conditions that might benefit from drug-induced memory enhancement”.

Furthermore, another study published in the American Journal of Psychiatry sought to test the effect of methylene blue on long-term memory.  Specifically, its effect on fear extinction and contextual memory. This was put to test by placing participants of the trial in a dark room after giving them a dose or placebo of methylene blue. The dark room was supposed to help augment their personal fears. The results of this study suggested that after a month of methylene e blue doses participants experienced a decrease in fear retention. Therefore, the study authors concluded, “Methylene Blue enhances memory and the retention of fear extinction”.

Methylene Blue helps with Bipolar Disorder

The use of methylene blue in the treatment of psychiatric conditions has been around since the early 1950s. Now we have evergrowing evidence that methylene blue has a positive effect on a well-known psychiatric condition called Bipolar Disorder. However, this study which was conducted at  Dalhousie University in Halifax has only used methylene blue in concurrence with another anti-psychotic medicine called Lamotrigine. In this study subjects with a known case of Bipolar Disorder were given lamotrigine (Lamictal®) along with daily supplementation of either 195 mg or 15 mg of Methylene Blue. At the end of the study, the results concluded that the patients receiving a higher dose of methylene blue along with Lamotrigine had reduced depression and anxiety-induced symptoms. The patients believe it was so effective that they chose to carry on with the methylene blue supplementation even after the trial was over.

Methylene Blue nootropic dosage

Although recommended dosages differ from guideline to guideline, most agree on 0.5 – 4 mg/kg. So a 90 kg (200 lb.) body weight translates to 45 – 360 mg of Methylene Blue. This was deduced after a significant amount of animal and human trials.

However, your physician may recommend that you start at the lowest dose of 0.5 mg/kg and see which dosage suits you after seeing best and how your body and mind react to it.

In case you still want to start at high doses to amplify the effects, we recommend a dose of 10mg daily. This should be split up between the morning and night. A good route to consume it would be to take it with juice or milk.

Methylene Blue is a water-soluble compound which means when taken orally, you don’t need to take the supplement in relation to a meal which is a contrast compared to other Nootropics. Methylene blue also has a half-life of about 5 hours which is why it’s okay to take up to two doses a day

One thing to keep in mind whilst taking this supplement is that it may turn your urine blue, but it is nothing to worry about as it only happens if your daily dosage exceeds 500 mcg. But if it still bothers you, a hack to avoid blue urine is to take Methylene Blue with Ascorbic acid, commonly known as vitamin C.

Methylene blue supplementations are usually oral however it does come in an injectable form as well. But this can only be prescribed by a physician and is usually only used to treat diseases like malaria or as a part of a chemotherapy regime. . Alternatively, methylene blue can be taken in many forms including orally dissolved in liquid, powder in a capsule and topically applied on your skin.

Liposomal Filtration

After the previous phase has been completed, we are left with a solution of various sizes and varieties of liposomes as well as byproducts and additives added during the liposome generation. This is why a filtration phase is necessary to sort the liposomes by their different varieties and exclude the byproducts. Usually, a selective filtration method is used by large ultrafiltration skids or tangential flow filtration systems. These methods use a selective membrane to filter out the liposomes. The latter of the two methods is more favorable as it is less time-consuming. It is compounded very well with microchip and jet injection methods in the liposomal generation phase as these methods produce smaller volumes of solvent and tangential flow filtration works best with smaller solvent volumes.

Liposomal Stabilization

After the filtration phase, we are usually left with a solution of a specific variety of liposomes in a solution of sodium chloride water. The solution is then further diluted to further eliminate any remaining byproducts. This phase also uses a variety of filters but usually, they are of the disposable kind. This is a tedious process as it usually takes a long time. After it is completed the liposome solution is refrigerated to prevent any form of degradation. Nowadays, products are always refrigerated between different phases of manufacturing.

Liposomal Loading

This phase of the manufacturing process is said to be the most chemically complex as we ll a significant step. This is the step that comprises the injection of the active ingredient into the carefully formed liposomes. Traditionally, the previously formed and filtered liposomes were heated at high temperatures to induce a chemical change in the pH so that the aqueous interior of the liposomes is exposed for the insertion of the active ingredient. The active ingredient easily penetrates the bilayer of phospholipids. This is of course carried out under extreme observation to ensure the potency of the end product. Once the active ingredient has melded with the layer the solution is then cooled down again to bring it to its former appearance of the liposomal structure, the only difference now is that the active ingredient is inside the liposomal spheres. After this is done some chemicals, like amino acids, sugars, PEG, and other chemicals used to control biophysical assimilation and stability, are used to attract the liposome to the target area of the body. These chemicals are used to count the outside of the liposomal spheres by rapidly heating and then cooling the solution causing pH changes for integration.